Spermac Stain é um método simples para a investigação detalhada da morfologia de todas as partes dos espermatozóides.

 

Coloração da morfologia do esperma

A avaliação da morfologia do esperma é um procedimento padrão na análise do sêmen. O método Spermac Stain é simples, mas perfeitamente adequado para a investigação detalhada da morfologia de todas as partes dos espermatozóides.

 

Os esfregaços corados são avaliados sob luz direta e imersão em óleo (1000x).

 

Informações sobre a composição do produto podem ser encontradas na ficha de dados de segurança do material.

 

Regulatório

 

Europa: Marca CE (IVDR) – EUA: registrado – Canadá: registrado

Brasil: registrado – Austrália: registrado

 

Códigos de pedido de produto

SPS050: Coloração de esperma – 4 x 50mL

SPS250: Coloração Espermac – 4 x 250mL

 

Interpretação dos resultados

Imagens do Prof. Philip Chan (Loma Linda Univ. Gyn/Ob-IVF, EUA)

 

Literatura do Produto

Önel T., Ayla S., Keskin I., et al., Leptin in sperm analysis can be a new indicator, Acta Histochemica (2019),Vol.121,pp.43-49

 

Afsin M., Otludil B., Dede O., Akkus M., An examination on composition of spermatozoa obtained from pre-operative and post-operative varicocele patients, Reproductive Biology (2018),Vol.18,pp.361-367

 

Bencharif D., Amirat, L., Pascal O., et al. , The advantages of combining low-density lipoproteins with glutamine for cryopreservation of canine semen., Reprod Domest Anim (2010),Vol.45,No.2,pp.189-200

 

Biricik A., Guney I., Berkil H., et al, A male (15;15) Robertsonian translocation case with 11 previous consecutive recurrent spontaneous abortions, Marmara Medical Journal (2004),Vol.17,No.1,pp.35-38

 

Cipak A., Stanic P., Duric K., et al. , Sperm morphology assessment according to WHO and strict criteria: method comparison and intra-laboratory variability., Biochemica Medica (2009),Vol.19,No.1,pp.87-94

 

El-Harouny M.A., Zalata A.A., Naser M.E., et al., Long-term ofloxacin testicular toxicity: an experimental study., Andrologia (2010),Vol.42,No.2,pp.92-96

 

Levitas E., Lunenfeld E., Weisz N., et al., Relationship between sexual abstinence duration and the acrosome index in teratozoospermic semen: analysis of 1800 semen samples, Andrologia (2006),Vol.38,pp.110-112

 

Levitas E., Lunenfeld E., Weisz N., et al., Relationship between age and semen parameters in men with normal sperm concentration: analysis of 6022 semen samples, Andrologia (2007),Vol.39,pp.45-50

 

Lockyear K.M., Goodrowe K.L., Waddell W.T. and MacDonald S.E. , Comparison of different osmolalities and egg-yolk composition in processing media for the cryopreservation of red wolf (Canis rufus) sperm., Theriogenology (2009),Vol.71,No.3,pp.469-479

 

Lukanov T.H., Lichev D.I., Konova E.I., et al. , Flow cytometric measurement of sperm nuclear DNA fragmentation in infertile men with normal standard sperm parameters., Journal of Mens Health (2009),Vol.6,No.1,pp.50-55

 

Miller J.E., Smith T.T., The effect of intracytoplasmic sperm injection and semen parameters on blastocyst development in vitro, Human Reproduction (2001),Vol.16,No.5,pp.918-924

 

Parapanov RN, Nusslé S., Crausaz M., et al., Testis size, sperm characteristics and testosterone concentrations in four species of shrews (Mammalia, Soricidae), Animal Reproduction Science (2009),Vol.114,pp.269-278

 

Rota A., Magelli C., Panzani D., Camillo F., Effect of extender, centrifugation and removal of seminal plasma on cooled-preserved Amiata donkey spermatozoa, Theriogenology (2008),Vol.69,pp.176-185

 

Rota A., Milani C., Romagnoli S., Effect of post-thaw dilution with autologous prostatic fluid on dog semen motility and sperm acrosome status, Theriogenology (2007),Vol.67,pp.520-525

 

Salumets A., Effects of embryological parameters on the success of fresh and frozen embryo transfers, pHD dissertation. University of Helsinki, Tartu University Press, Finland (2003),Vol.1

 

Soygur B., Celik S., Celik-Ozenci C., Sati L., Effect of erythrocyte-sperm seperation medium on nuclear, acrosomal, and membrane maturity parameters in human sperm, J Assist Reprod Genet (2018),Vol.35,pp.491-501

 

Svalander P., Jakobsson A-H., Forsberg A-S., et al., The outcome of intracytoplasmic sperm injection is unrelated to ‘strict criteria’ sperm morphology, Human Reproduction (1996),Vol.11,No.5,pp.1019-1022

 

Webb G.W., Dean M.M. , Effect of Centrifugation Technique on Post-storage Characteristics of Stallion Spermatozoa., Journal of Equine Veterinary Science (2009),Vol.29,No.9,pp.675-680

 

Yakin K., Balaban B., Urman B., Is there a possible correlation between chromosomal variants and spermatogenesis?, International Journal of Urology (2005),Vol.12,pp.984-989

 

Zalata A., Elhanbly S., Abdalla H., et al. , In vitro study of cypermethrin on human spermatozoa and the possible protective role of vitamins C and E., Andrologia (2014),Vol.46,No.10,pp.1141-1147

 

Zalata A.A., Hassan A.H., Nada H.A., et al., Follicle-stimulating hormone receptor polymorphism and seminal anti-Mullerian hormone in fertile and infertile men., Andrologia (2008),Vol.40,No.6,pp.392-397

 

Zalata A.A., Morsy, H.K., Badawy Ael N., et al. , ACE gene insertion/deletion polymorphism seminal associations in infertile men., J Urol (2012),Vol.187,No.5,pp.1776-1780